Continuing its action in conservation, Bionesia was again held another workshop entitled “Basic Molecular Training on DNA Forensic of Sea Turtle”. This workshop was a continuation of the Sea Turtle Project of Bionesia which was funded by Rufford Foundation (Grant ref. no: 22043-1) . Our first workshop in this Project was held in Turtle Conservation and Education Center (TCEC) – Serangan, Bali on July 29, 2017, entitling Stranded Sea Turtle and Application of Forensic DNA in Sea Turtle Conservation.
On this second workshop, it was held in Magister Program of Udayana University on September 18-19, 2017 and collaborated with Magister of Biology, Udayana University and WWF-Indonesia. Eleven different faculties from public and private universities around Bali were invited in this workshop including Udayana University, IKIP PGRI Bali, Universitas Hindu Indonesia, Universitas Mahasaraswati Denpasar, Universitas Pendidikan Ganesha, & Universitas Dhyana Pura. Three government institutions and one NGO were also invited, including BPSPL Denpasar, Loka Tuna Fisheries Research Denpasar, Fish Quarantine and Inspection Agency & TCEC-Bali. Among all the participants, most of them are lecturer and some of which have not used molecular genetic in their research study. Therefore, such training is a very good improvement of knowledge and science for their curriculum.
Ni Putu Dian Pertiwi, one of the instructor was giving explanation about PCR (Polymerase Chain Reaction), one of the method used in molecular genetic
During this workshop, the participants were introducing to the use of molecular DNA as a tool for forensic identification, which mainly in the form of biological product that was sell and trade illegally. Samples that were used are the tissue from sea turtle cloaca, sea turtle meat, shark meat and shark fin. Introduction to the molecular techniques was given in class and laboratory hands on experience, which include DNA extraction, PCR, electrophoresis and data analysis. Extraction is a method to isolate and extract DNA from the tissue sample. PCR is an abbreviated for Polymerase Chain Reaction, a method to amplify target DNA. Electrophoresis is a technique to visualize the result of PCR product using gel and electrical current. Other than those laboratory techniques, participants were also introduce to the data analysis method and how to interpret the result of the sequence data. All of the class materials and discussion were given by the instructors from BIONESIA (Andrianus Sembiring, Ni Putu Dian Pertiwi, Astria Yusmalinda) and Udayana University (I Nyoman Giri Putra, Maulid Dio Suhendro).
The situation of laboratory activity. Each participant were given a chance to do the genetic lab-work
Through this event, it is expected to increase the enthusiasm and potential of each of the participant to develop and expand the possibility of molecular study and conservation of wild animals in Bali and Indonesia. The participants were also excited to share their experiences in sea turtle conservation happened in their area, and some of them were also discuss their research study related to sea turtle and how they can combine it with molecular techniques. Bionesia was also had a chance to increase its collaboration with the universities being the participants in this training through MoU agreement. The collaboration includes internship for the student of the universities into BIONESIA lab and research collaboration between two institutions.