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Microsatellite DNA Training in Loka Tuna Fisheries Research

Posted on Posted in Capacity Building, Workshop

By: Ni Putu Dian Pertiwi & M. Danie Al Malik

On November 16, 2018 Bionesia was invited by the Loka Tuna Fisheries Research in Denpasar-Bali to give training on microsatellite data analysis. The aim of this training is to share knowledge about how to perform microsatellite fragment data analysis, and interpret the result, using several analysis software, such as genAlx, geneious, arlequin, etc. During this event, Astria Yusmalinda, one of Bionesia’s staff who is previously worked with microsatellite analysis of Napoleon wrasse, was invited to be the trainer.

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Our Staff Astria Yusmalinda is giving an explanation on how to do data interpretation for the microsatellite result.

Microsatellite is one of the important tools in genome mapping, and uses simple sequence tandem repeats (SSTRs) as the marker. This tract of repetitive DNA has a higher mutation rate than other areas of DNA leading to high genetic diversity. This method has various applications, including for cancer diagnosis, forensic and medical fingerprinting, paternity testing, population genetic, and plant breeding.

Within this training, the use of microsatellite was more focusing for identifying population structure of tuna fishes in Indonesian water, which was one of the main researches of Loka Tuna Fisheries Research Denpasar. Nine participants were involved in the training with 8 researchers staff of Loka Tuna Denpasar, and one undergraduate students of Marine Science Dept., Udayana University. As the trainer, Astria Yusmallinda was hoping that the knowledge sharing through this training could be useful for the participants, thus they will be able to use this method for their current research.