By: M. Danie Al Malik and Ni Putu Dian Pertiwi
On October 4-6, 2019, Magister Program of Udayana University (UNUD) and Yayasan Biodiversitas Indonesia (BIONESIA), collaborated with Indonesian Biodiversity research Center (IBRC) and Marine Biodiversity Project (DMBP)-Diponegoro University held a biodiversity assesstment workshop entitled “Advance Molecular Genetic Methods for Biodiversity Assessment”. This workshop is part of the PEER Science project supported by USAID. The workshop located at IBRC’s Marine Field Station, Pemuteran-Bali, and aims to introduce the techniques to assess and measure biodiversity. It also aims to introduce the newest molecular genetic methods called Next Generation Sequencing (NGS). Participants of this training program were the students, staff, and lecturer of Udayana University, and also researchers from several local institutions, including Loka Tuna Fisheries Research, Balai Besar Penelitian dan Pengembangan Budidaya Laut-Gondol (BBPPBL-Gondol) and West Bali National Park (TNBB).
Gondol research staff is explaining about the fish farming procedure for abalone
The workshop is divided into different agendas, including visitation to Gondol research center (BBPPBL-Gondol), class study, field sampling, samples preparation and preservation. Class study materials were given by the research staff of BIONESIA. On the first day of the workshop, the participants visited Gondol Research Center and introduced about the marine farming techniques on several organisms, including abalone, clam, sea cucumber, grouper and tuna. On the next day, the participants were invited to participate in the field activities, such as ARMS (Autonomous Reef Monitoring Structures) recovery and eDNA sampling.
The participants were learn on how to collect samples from beach sand for the eDNA purposes.
The ARMS were previously deployed at the depth of 10 m at the beach near Biorock, Pemuteran-Bali, and left there for almost 3 years. Meanwhile, as for the eDNA sampling, the participants were also collected the samples from the beach and sea water near Biorock. On the laboratory activities, participants were introduced to the techniques used to collect biota living on the ARMS, and also how to preserve samples from ARMS and eDNA. The samples were then can be used for further analysis using NGS methods.
Meanwhile, the class presentation were including introduction to ARMS, NGS and its application for research study, program for analyzing benthic percentage cover (CoralNet), and also introduction of system database to store biodiversity information (GeOME- Observatories MetaDatabase).
Ni Putu Dian Pertiwi (BIONESIA) is presenting a class material about NGS
The opening and closing ceremony of this workshop was attend by the Director of Magister Program of Udayana University, Prof. Dr. dr. I Putu Gede Adiatmika, M.Kes. He said that this type of workshop and training will help students, staff, lecturer and also researchers to learn and understand the most update technologies for biodiversity research and can open the possibilities for research collaborations.